Survey Snippets: Calibration and Reference Materials for Cytometer

Survey Snippets: Calibration and Reference Materials for Cytometer Measurement “Standardization” NIST-FDA Flow Cytometry Workshop: Building Measurement Assurance in Flow Cytometry

Wide range of fluorescent parameter is used

Several approaches to QC the flow cytometer (w/o further calibration/characterization) (w further calibration/characterization)

No consensus approach for QC and performance characterization (to NIST standards) (to mfg assigned intensities)

No consensus on how to perform cytometer compensation

No consensus about the compatibility/comparability of results from flow

Some efforts to perform inter-laboratory flow cytometry studies

Discussion topics Reference materials for 12 color flow (calibration/compensation)? Comparability between instruments? Reliance on MFG calibration? What are home-built protocols/materials based on?