Rocky Mountains Conference EPR Workshop: DEER Analysis

Rocky Mountains Conference EPR Workshop: DEER Analysis programs testing

DEFit (for CW spectra: CwdipFit) Sen I., Logan, T. and Fajer P., Biochemistry, 2007;46:11639-49. http://fajerpc.magnet.fsu.edu/Programs/DEFit/defit.html

All spectra were provided by the anonymous symposium participants and analyzed blindly using DEFit (till today we don’t know what the spectra are) The spectrum naming (slide titles) follow the key provided by the panel moderator, Dr. Eric Hustedt. Hardware: Intel Duo Core2, Matlab 2007b

2a 3 Gaussians C A,B components A and B very well defined: narrow distributions assymetric population component (c) badly defined component

2b Noisy data large error surface average distance better defined than the distribution

data0001 Excellent definition of three populations complex assymetric population

data0002 one population, very well defined

data0003 components A and B very well defined: narrow distributions assymetric population component (c) badly defined component

last0001

last0002 Spikes are not always spikes! two components one very narrow

last0003 two components one very narrow

last0004

DATA1 low modulation depth, very long distance and very broad

DATA2 three components, chi2 impovement 6x from 2 components. one extremely sharp